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Part:BBa_K3733032:Experience

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)


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Applications of BBa_K3733032

We transform E.coli DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1. OD600 corrected RFU values culturing after 12 h after induction of different concentrations of sodium thiosulfate

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